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CDKN2A/p19ARF Rabbit pAb(bs-11

74R)-50ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-1174R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1174R
    英文名称CDKN2A/p19ARF Rabbit pAb
    中文名称抑癌基因p19抗体
    英文别名CD2A2_HUMAN; ARF; Cyclin dependent kinase inhibitor 2A; INK4; MTS1; P19.
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Rat (Mouse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from mouse CDKN2A/p19ARF
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量19 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cell cycle > Cell cycle inhibitors > Ink4

    Cancer > Oncoproteins/suppressors > Tumor suppressors > p53 pathway

    Cell Biology > Cell Cycle > Cell Cycle Inhibitors > Ink4

    Epigenetics and Nuclear Signaling > Cell cycle > Cell Cycle Inhibitors > Ink4

    Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Tumor Suppressors

    亚基Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts (isoforms 1,2 and 4) with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2.
    亚细胞定位Cytoplasm. Nucleus.
    组织特异性Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.
    翻译后修饰Ubiquitinated in normal cells by TRIP12 via the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination at the N-terminus, regardeless of the absence of lysine residues. Ubiquitination leads to its degradation. In cancer cells, however, TRIP12 is located in a different cell compartment, preventing ubiquitination and degradation.
    相似性Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.
    功能Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012].

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩?? for 20 min;
    Incubation: Anti-CD2A2 Polyclonal Antibody, Unconjugated(bs-1174R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    Tissue/cell: Rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-CD2A2 Polyclonal Antibody, Unconjugated(bs-1174R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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    图标文献和实验
    该产品被引用文献

    [IF={{ 2.33 }}] {Liang, Wu, et al. "Knockdown BMI1 expression inhibits proliferation and invasion in human bladder cancer T24 cells." Molecular and cellular biochemistry (2013): 1-9.} {WB} {="Human"}

    [IF={{ 12.5 }}] {Wang Simeng. et al. Loss of CDKN2A Enhances the Efficacy of Immunotherapy in EGFR Mutant Non-Small Cell Lung Cancer. CANCER RES. 2024 Nov;:} {WB} {}

    [IF={{ 12.5 }}] {Simeng Wang. et al.Loss of CDKN2A Enhances the Efficacy of Immunotherapy in EGFR-Mutant Non–Small Cell Lung Cancer.cancer research.2025 Feb 1;85(3):585-601.} {Western blot} {Mouse}

    相关实验

    • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

      = NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

    • secondary antibody review -- data from 99 publications

      data

    • 消化病学领域内基因芯片技术的应用

      结肠肿瘤激光微切获得的肿瘤组织中，p53基因的外显子5～7在多重肿瘤细胞中是正常的。提示在交互阅读框(ARF)-p53途径中高水平的ARF足以稳定p53，使其不发挥生物效应，致癌基因的作用机制可能在ARF-p53通路以上。在结肠癌发病机制研究方面，Suzuki等采用cDNA芯片筛查了经甲基化和抑制组蛋白乙酰化后的结肠癌细胞株基因表达水平的变化，结果发现基因启动子去甲基化和抑制组蛋白乙酰化后的基因表达上调；高甲基化的基因家族表达下调。不但证实抑癌基因启动子甲基化和组蛋白乙酰化是导致结肠癌发生的重要机制

    查看更多相关实验 >
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    文献支持
    CDKN2A/p19ARF Rabbit pAb(bs-1174R)-50ul/100ul/200ul
    ￥1180 - 2800