Sample: A549 Cell Lysate at 40 ug
Primary: Anti-ostepontin (bs-0019R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 50 kD
- ￥1180 - 2800
- Bioss
- bs-0019R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0019R |
| 英文名称 | Osteopontin Rabbit pAb |
| 中文名称 | 骨桥蛋白/分泌型磷蛋白1抗体 |
| 英文别名 | BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:500-2000, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human OPN |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 34 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Ligand for integrin alpha-V/beta-3. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Bone. Found in plasma. |
| 相似性 | Belongs to the osteopontin family. |
| 功能 | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:500-2000} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-Osteopontin (bs-0052R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 60 kD
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-Osteopontin (bs-0052R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Polyclonal Antibody, Unconjugated (bs-0019R) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (bs-0019R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (bs-0019R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 5.9 }}] {Tainara C. Michelotti. et al. Subclinical ketosis in postpartum dairy cows alters the adipose tissue immunological profile in a depot-specific manner. FRONT IMMUNOL. 2025 Jun;16:} {IHC} {Bovine}
[IF={{ 3.8 }}] {Hongrong Zhang. et al. Extracellular matrix of ameloblastoma-derived negatively regulates osteogenic differentiation. ORAL DIS. 2023 Jul;:} {WB} {Human}
[IF={{ 3.539 }}] {Shunli Feng. et al. SPP1 as a key gene in the lymph node metastasis and a potential predictor of poor prognosis in head and neck carcinoma. J ORAL PATHOL MED. 2022 Jul 13} {WB,IHC} {Human}
[IF={{ 3.23 }}] {Liu C, Zhang Y, Wang L, Zhang X, Chen Q, Wu B (2015) A Strontium-Modified Titanium Surface Produced by a New Method and Its Biocompatibility In Vitro. PLoS ONE 10(11): e0140669.} {WB} {="Rat"}
[IF={{ 3.08 }}] {Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).} {WB} {="Human"}
相关实验
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基因-2(BCL-2) ELISA 48T/96T 1500/2500 进口分装 F0017 脑衍化神经营养因子(BDNF) ELISA 48T/96T 1800/2800 进口分装 F0018 骨成型蛋白-2(BMP-2) ELISA 48T/96T 2000/3000 进口分装 F0019 骨成型蛋白-4(BMP-4) ELISA 48T/96T 2000/3000 进口分装 F0020 骨成型蛋白-7(BMP-7) ELISA 48T
基因-2(BCL-2) ELISA 48T/96T 1500/2500 进口分装 F0017 脑衍化神经营养因子(BDNF) ELISA 48T/96T 1800/2800 进口分装 F0018 骨成型蛋白-2(BMP-2) ELISA 48T/96T 2000/3000 进口分装 F0019 骨成型蛋白-4(BMP-4) ELISA 48T/96T 2000/3000 进口分装 F0020 骨成型蛋白-7(BMP-7) ELISA 48T
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Osteopontin Rabbit pAb(bs-0019R)-50ul/100ul/200ul
￥1180 - 2800
