Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, H1299, 293T, C2C12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
- ￥1580 - 2500
- Bioss
- bs-60163R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1580.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 产品编号 | bs-60163R |
| 英文名称 | Mono-Methyl-Histone H4 (Arg19) Rabbit pAb |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat, Rice |
| 抗体来源 | Rabbit |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | Antigen affinity purification |
| 克隆类型 | Polyclonal |
| 理论分子量 | 11 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Histones > H4 > Acetylated |
| 亚基 | The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. |
| 亚细胞定位 | Nucleus. Chromosome. |
| 翻译后修饰 | Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. |
| 相似性 | Belongs to the histone H4 family. |
| 功能 | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验相关实验
Site-directed hydroxy radical mapping of nucleosome positions in vitro
and Davey, 2003). Procedure Preparation of Derivatised Octamer Refold and purify histone octamer containing histones H2A wild type, H2B wild type, H3 Cys110Ala and H4 Ser47Cys following standard methods (Dyer et al., 2004) (see note 1);
Isolation of Histones and Nucleosome Cores from Mammalian Cells
Simon, R.H. and Felsenfeld, G. 1979. A new procedure for purifying histone pairs H2A+H2B and H3+H4 from chromatin using hydroxylapatite. Nucl. Acids Res. 6:689‐696.
Reagents 100-bp DNA size ladder (Promega) Agarose Antisera (affinity-purified) These should be raised against histone peptides with mono-, di-, or trimethylation or acetylation at a specific lysine/arginine residue
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Mono-Methyl-Histone H4 (Arg19) Rabbit pAb(bs-60163R)-50ul/100ul
￥1580 - 2500
