Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(bs-0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-0498R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0498R |
| 英文名称 | ADRB1 Rabbit pAb |
| 中文名称 | 肾上腺素能受体β1抗体 |
| 英文别名 | beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; beta 1 adrenoceptor; beta 1 adrenoreceptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB1_HUMAN; ADRB1R; Adrenergic beta 1 receptor; Adrenoceptor beta 1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ADRB1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood > Blood Pressure regulation Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Adrenergic Receptors Signal Transduction > Growth Factors/Hormones > Adrenalin Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚基 | Interacts with GOPC, MAGI3 and DLG4. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. |
| 翻译后修饰 | Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family.
Adrenergic receptor subfamily. ADRB1 sub-subfamily. |
| 功能 | Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Tissue/cell: skin of rat foot; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(bs-0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(bs-0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Heart (Mouse) Lysate at 30 ug
Heart (Rat) Lysate at 30 ug
Primary: Anti- ADRB1(bs-0498R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 52 kD
Observed band size: 55 kD
Heart (Mouse) Lysate at 30 ug
Heart (Rat) Lysate at 30 ug
Primary: Anti- ADRB1(bs-0498R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 52 kD
Observed band size: 55 kD
Tissue/cell: mouse kidney cell;
Incubation:
Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated(bs-0498R-FITC) 1:200, 40 minutes at 37°C.
Green line: Isotype Control(bs-0295P-FITC, rabbit IgG/FITC) 1:200, 40 minutes at 37°C.
Incubation:
Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated(bs-0498R-FITC) 1:200, 40 minutes at 37°C.
Green line: Isotype Control(bs-0295P-FITC, rabbit IgG/FITC) 1:200, 40 minutes at 37°C.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (bs-0498R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (bs-0498R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti- antibody (bs-0498R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti- antibody (bs-0498R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 6.208 }}] {Lina S. Farhoumand. et al. Blockade of ß-Adrenergic Receptors by Nebivolol Enables Tumor Control Potential for Uveal Melanoma in 3D Tumor Spheroids and 2D Cultures. INT J MOL SCI. 2023 Jan;24(6):5894} {FCM} {Human}
[IF={{ 4.831 }}] {Liu Z et al. Over-expression of microRNA-145 drives alterations in β-adrenergic signaling and attenuates cardiac remodeling in heart failure post myocardial infarction. Aging (Albany NY). 2020 Jun 18;12(12):11603-11622.} {WB} {Rat}
[IF={{ 3.727 }}] {Yue Wang. et al. Mirabegron Ameliorated Atherosclerosis of ApoE−/− Mice in Chronic Intermittent Hypoxia but Not in Normoxia. 2021 Jun 21} {IHC} {Mouse}
[IF={{ 3.14 }}] {Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.} {WB} {rat}
[IF={{ 3.07 }}] {Deng, Guo-Hua, et al. "Exogenous norepinephrine attenuates the efficacy of sunitinib in a mouse cancer model." Journal of Experimental & Clinical Cancer Research 33:21 (2014).} {IHC} {="Mouse"}
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ADRB1 Rabbit pAb(bs-0498R)-50ul/100ul/200ul
￥1180 - 2800
