Blank control:A375.
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Primary Antibody (green line): Mouse Anti-human CD73 antibody (bsm-30125M)
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Dilution: 1ug/Test;
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Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
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Dilution: 0.5ug/Test.
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Isotype control（orange line）：Normal Mouse IgG
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Protocol
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The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1880
- Bioss
- bsm-30125M
- 2026年10月17日
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- 技术资料
- 规格：
100ug
| 产品编号 | bsm-30125M |
| 英文名称 | human CD73 Mouse mAb |
| 中文名称 | 人CD73单克隆抗体 |
| 英文别名 | NT5; 5' NT; 5' nucleotidase(CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto(CD73); 5'-NT; 5'-nucleotidase; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN antibody eNT; NT antibody NT5; NT5E; NTE; Purine 5 Prime Nucleotidase. |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 亚型 | Mouse IgG1, k |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 63 kDa |
| 实际分子量 | 70kDa |
| 储存液 | 0.01M TBS (pH7.4). |
| 研究领域 | Cardiovascular > Atherosclerosis > Vascular Inflammation > Leukocyte recruitment Cardiovascular > Hypoxia > Associated Proteins |
| 亚细胞定位 | Cell membrane. |
| 相似性 | Belongs to the 5'-nucleotidase family. |
| 功能 | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
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NT5E | Human CD73 Mouse mAb(bsm-30125M)-100ug
￥1880
