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文献和实验Cell Reports|浙大陈晓团队解码肌腱成熟过程的关键细胞图谱
.com/cell-reports/fulltext/S2211-1247(22)00526-5
The Effect of Caffeine on the Heart Rates of Three-Day Old Chick Embryos
of caffeine to which the heart was exposed. Procedure Preparation: Prep sheet 1) Warm Dulbecco's modified Eagle Medium (DMEM, Sigma) and Howard's Ringers solution to 37 degrees C. Diliute the 1 mg/ml stock caffeine solution in DMEM. 2) Pour
at least 2 mg (up to 4mg) of total RNA in 750 l RNase-free water. In general, inputting more total RNA will yield more polyA RNA. This protocol is most successful with inputs of at least 2 mgs. (Less than 1mg of total RNA input is NOT recommended
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