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- 2025年10月24日
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999
- 供应商：
联硕生物
- 规格：
250MG
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文献和实验Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
temperature for 4 hours and then dehydrated through a graded series of ethyl alcohols: 50% and 70% in the refrigerator (4 °C), and 85%, 95% (3 changes), and 100% (4 to 5 changes) in room temperature. The brain is left in each solution for 24 hours.
Protein Expression and Purification of RPA70-AB (181-422)
Comments: pSV281 RPA70AB Constructed by L.Mizoue; May '02.This plasmid encodes RPA 70 subunit residues 181-422 as a fusion protein with an NT 6His tag.The tag can be cleaved off with TEV protease (recognition site is ENLFYQG)leaving "GS" at NT
filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
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