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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
250MG

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文献和实验一、 试剂制备 MES缓冲液 250ml MES 0.1mol/L 4.88g EGTA 1.0mmol/L 95.09mg Mgcl2 0.5mmol/L 25.41mg PH 6.5 临用前加GTP or ATP 1.0mmol/L 4℃保存 微管聚合缓冲液 250ml MES 0.1mol/L 4.88g EGTA 1.0mmol/L 95.09mg Mgcl2 0.5mmol/L 25.41mg Glycerin 8mmol/L 184.18mg PH
medium : sucrose 250 mM, EDTA 2 mM, BSA 1 mg/ml, pH 7.4) (2) The pellet was re-suspended in 1ml of Grinding medium and sonicated. (3) Nuclei were collected by centrifugation for 12 min at 800g at 4℃ and the nuclear proteins were extracted
filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
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