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- Sigma
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- S531057-250MG
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
250MG
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro
In Situ HYBRIDIZATION TO TISSUE SECTIONS
dechorionated embryos to either buffer A or B (depending on their age): Buffer A (0-2, 1-3 hr embryos) 2.5 ml 8% paraformaldehyde (fresh) 2.0 ml 1X PBS 0.5 ml DMSO - or - Buffer B (2-4, 3-5
HIGH RESOLUTION GENETIC FOOTPRINTING
volume (final concentration, amount of stock added to make 250 ml solution) 50 mM Tris pH 8.0, 12.5 ml of 1 M stock 10 mM EDTA, 5 ml of 0.5 M stock 140 mM NaCl, 7 ml of 5 M stock 10% glycerol, 31.25 ml of 80% stock 10 mM DTT, 385 mg 1 mM PMSF, 4.35 ml
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