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文献和实验, especially when focusing in the very basic or acid pH region.The higher the pH dependence of the mobility, (dµ /dpH), the better the focusing. A high electrophoretic mobility close to the IpH will efficiently transfer diffused protein molecule back to IpH
of each component. 2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly
Assays of d-Amino Acid Oxidases
d -Amino acid oxidase and d -aspartate oxidase are two well-known FAD-containing flavooxidases that catalyze the same reaction (the oxidative deamination) on different d -amino acids. d -aspartate oxidase is specific for acidic d -amino
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