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- 2025年10月24日
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- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
500ML
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文献和实验to individual reactions USING PLUGGED TIPS.OVERLAY REACTIONS WITH 50UL OF HIGH-QUALITY LIQUID PARAFFIN OR MINERAL OIL to ensure no evaporation occurs: this changes reactant concentrations. NOTE: latest wisdom has it one can use VASELINE - this also allows "HOT
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
PROTEIN PURIFICATION 1) grow 20ml cells O/N 37 C, dilute 50X into prewarmed LB, grow to 0.6 OD or about 1hr. Induce w/ 2mM IPTG (238mg/0.5l), grow 3hr, spin 6k GS3 10'', freeze at -70 C. 2) extract cells (from 500ml) in 25 mls. Heintz Buffer
: 72°C standard temp. and est. time based on length of template (approx. 1 min per 1 kb) Analyze 10 ml of each sample on a 3.0% Metaphor agarose gel, or a 6% acrylamide gel. Be sure to run Sty I or some other molecular weight marker
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