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- BML00125-1G
- 2025年10月24日
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文献和实验Preparation of probes for in situ hybridization
fluorophores: The nucleic acid to be labelled should be resuspended in 70 µl of 0.1 M NaHCO3 buffer, pH 8.8. Different amounts of material will result in different specific activities of the probe. 5-10 µg of nucleic acid will yield a high specific
Preparation of probes for in situ hybridization
in 70 µl of 0.1 M NaHCO3 buffer, pH 8.8. Different amounts of material will result in different specific activities of the probe. 5-10 µg of nucleic acid will yield a high specific acitity (60-80% of the free amine will react), while 50 µg will yield
; dT, Cat. No. 10-1030-10) and an amino-allyl modified base for attachment of ester-conjugated fluorophores (C6-dT, Cat. No. 10-1039-05) were obtained from Glen Research. Oligonucleotide Purification Cartridges (OPCTM, Cat. No. 400771) and 2M
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