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- 2025年10月24日
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鹿森生物
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10MG
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文献和实验Colloidal Gold/Streptavidin Methods
. This nonspecific binding has been attributed both to its high isoelectric point (PI = 10) and to the fact that the protein is glycosylated (3 , 4 ). Because of the problem of nonspecific binding of avidin, streptavidin has largely replaced avidin
Smart Polymer-Streptavidin Conjugates
The conjugation of stimuli-responsive, or “smart,” polymers to streptavidin is described. The polymer is synthesized with a thiol-reactive end-group, which is used to end-graft the polymer to cysteine or lysine side-chains
Covalent Coupling of DNA Oligonucleotides and Streptavidin
Semisynthetic DNA-protein conjugates are synthesized by covalent coupling of thiol-modified DNA oligonucleotides and streptavidin. The resulting conjugates have a binding capacity for four equivalents of biotin and one nucleic acid
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