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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
1ML

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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
Peroxidase Conjugation by Periodate Method
Reagents Horseradish peroxidase Sodium periodate, 0.1M, freshly made Ethylene glycol Sephadex G-25 Sodium acetate buffer, 0.001M, pH 4.2 IgG Carbonate:Bicarbonate Buffer, 1.0 M, pH 9.5 Sodium tetraborate (4 mg/ml) freshly
600 or 0.6 to 1.0. This represents a cell density of approximately 0.6 - 1 x 107 cell/ml. Wash the cells in 0.5 vol of 1.0 M. sorbitol, 10 mM Bicine-NaOH (pH 8.35), 3% ethylene glycol, 5% DMSO (Solution 1), and resuspend in 0.02 vol of the same solution. Freeze the 0.2
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