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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
10MG

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文献和实验Methylation of Fatty Acids (Kropinski Method)
of 400 nmoles/100ml (usually 10mg of C15 in 100ml of reagent. Weigh 10mg of whole cells or 1 mg of LPS into a clean screw-cap tube. Add 1ml of MeOH-HCl reagent/internal standard into each tube and vortex. Heat at 100o C for 20 minutes
Standard neutral agarose electrophoresis
8. 1 x TAE buffer - 40mM Tris-acetate, 2mM EDTA, pH 8. 10mg/ml ethidium bromide stock solution 10 x Type III loading buffer (50% glycerol, 0.5% bromophenol blue, 0.5% xylene cyanol, this solution should be stored at 4°C) N.B: Extreme caution
Purification of Myosin Light Chain Kinase
7.0 4.Buffer C DE 52 Elution 10 mM MOPS- 5ml of 2M stock solution 1 liter 1 mM EDTA- 5ml of 0.2M stock solution 200 mM MaCl- 11.7g 1 mM DTT* - 1ml of 1M stock solution 0.1 mM PMSF* - 1.75ml of 10mg/ml stock solution pH 7.0 5.Buffer D Dialysis 10 mM MOPS
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