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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
250G

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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
side, then setting the to duration and turning the appropriate dial until the desired number is displayed. Typical settings for GSS-250: 40 V, 30 microsec, 2 repeats, for GSS-500: 75 V, 35 microsec and for GSS-1000: 137 V, 26 microsec.The parameters
Nuclear fractionation protocol
HEPES, 1.5 mM MgCl2 , 0.2 mM EDTA, 0.5 mM DTT, 26% glycerol (v/v), pH 7.9 To prepare 250 ml stock of buffer B: HEPES: 1M = 238.3 g/L, therefore 5mM = 0.295 g/250 ml MgCl2 : 1M = 203.3 g/L, therefore 1.5mM = 0.076 g/250
NP40 = 0.05% (v/v) 3. Buffer C 5mM HEPES 1.5mM MgCl2 0.2mM EDTA 0.5mM DTT 26% glycerol (v/v) pH 7.9 4. To prepare 250ml stock of Buffer C: HEPES: 1M = 238.3g
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