- ￥99 - 999
- Sigma
- 进口
- I4632-100MG
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
100MG
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文献和实验RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
Preparation of Yeast DNA Embedded in Agarose Plugs
into each plug mold to harden. A 100 ml culture should yield approximately 20 plugs. Plugs may be allowed to set at room temperature or placed at 4o C (for 15 minutes). spheroplasting solution . Incubate at 37o C for 2-4 h with gentle shaking. Longer
Preparation of Yeast DNA Embedded in Agarose Plugs
Solution 1% lithium dodecyl sulfate 100 mM EDTA 10 mM Tris-HCl, pH 8.0 Per 1 liter: 10 g lithium dodecyl sulfate (Sigma Chemical Cat #L-4632) 200 ml 0.5M EDTA, pH8.0 10 ml 1M Tris-HCl, pH8.0
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