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- 2025年10月24日
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- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
25MG
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文献和实验Quick and simple mouse forebrain commissure anatomy
in distilled water; cool to 4C. b. 10% neutral buffered formalin (easiest) - to make 1L: 900mL distilled water 4g sodium phosphate monobasic + 6.5g sodium phosphate dibasic(anhydrous) 100mL stock 40% formaldehyde solution (contains methanol
at a time while mixing until the pH is ~6.7 add concentrated NaOH dropwise to achieve pH = 6.9 distilled water to 500 ml sterile filter and store at 4oC 0.5 M EDTA Stock* 16.81 g EDTA (Sodium Salt) distilled water to 90 ml Adjust pH
Growing feeder-independent embryonic stem cells§
medium (Sigma, G5154) supplemented with 2 mM glutamine (Gibco-BRL), 1 mM sodium pyruvate (Gibco-BRL), 1× nonessential amino acids, 10% (v/v) fetal bovine serum (characterized, Hyclone), a 1:1000 dilution of beta-mercaptoethanol stock solution, and 500
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