- ￥99 - 999
- Sigma
- 进口
- 69152-25G-F
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
25G
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文献和实验Xenograft Tumor Model Protocol
medium and wash cells with PBS. Add a minimum amount of trypsin-EDTA. Disperse cells and add complete medium (10:1 to 5:1). Centrifuge immediately at or below 1500 rpm for 2-5 min and wash twice with PBS and store cells on cell. Count cells using
1) Take one medium sized leaf or half a large leaf (5 to 20 cm^2), weigh and freeze in liquid nitrogen. 2) Grind the tissue in a bleached and baked pestle and mortar with liquid nitrogen. 3) Transfer the powder produced to a l5ml Falcon blue cap
produced to a l5ml Falcon blue cap tube. Add 2 ml of homogenization buffer (4 ml per g) and disperse the tissue in it. 4) Leave for 10 min at room temperature. 5) Add 2 ml of phenol/chloroform and vortex. OR, 1) Put a small or medium sized leaf
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