- ￥99 - 999
- Sigma
- 进口
- 68268-100ML-F
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- CAS号：
288-32-4
- 规格：
100ML
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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
CCMB buffer to make chemical competent cell
does not appear to be necessary Test competence (see below) Good cells should yield around 100 - 400 colonies 4. CCMB80 buffer 10 mM KOAc pH 7.0 (10 ml of a 1M stock/l) 80 mM CaCl2.2H2O (11.8 g/l) 20 mM MnCl2.4H2O (4.0 g/l) 10 mM MgCl2.6H2O (2.0 g/l) 10
Preparation of Modifed Radioimmunoprecipitation (RIPA) Buffer
for phosphatase assays .] Procedure Prepare 100 ml modified RIPA buffer as follows: 1. Add 790 mg Tris base to 75 ml distilled H2 O. Add 900 mg NaCl and stir the solution until all solids are dissolved. Using HCl, adjust the pH to 7.4. 2. Add
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