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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
25G

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文献和实验适用于,无师兄、师姐、非细心、单独开展细胞培养的实验者 对一般细胞 0.25% 胰酶(胰蛋白酶,Trypsin) 配方:100ml PBS,0.25g胰酶 步骤:1先配100mlPBS(1000mlPBS:8.0gNaCl,3.4785gPO4HNa2・12H2O /2.9gPO4HNa2 ,0.2gKCl,0.2gPO4H2K溶于1000ml蒸馏水) 2 称胰酶0.25g 3 加入PBS中,低速搅拌 〈4h冰浴中,或者4℃过夜 低速搅拌0.5h冰浴中
Purification of acidic phosphatase from mustard seedlings
. cylinder and note the volume. Remove 1 ml aliquot to an Eppendorf microfuge tube, label as SN2 and leave on ice. Resuspend the pellet in 2-3 ml of ice cold water. Label as P2 and freeze at -20 °C. 12. To the supernatant, add another 0.25g (NH4 )2 SO4 /ml
Purification of acidic phosphatase from mustard seedlings
and the cytosol) into a graduated cylinder and note the volume. Remove 1 ml aliquot to an Eppendorf microfuge tube, and label as SN1 and leave on ice. This will be used for subsequent protein and activity determination. Later freeze at -20 °C. Resuspend the pellet
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