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文献和实验serum, 5% (vol/vol) fetal calf serum, 4 mM glutamine and 100 U ml−1 each of penicillin and streptomycin. It can be stored at 4 °C and used within 1�2 weeks after preparation. Sucrose buffer The solution
Lipoprotein Isolation - First week
, refractometry and dialysis. Day 3 Lyophilization. Day 1: Bleeding and centrifugation. Note: You need to wear gloves throughout the procedure. Each groups takes 10-15 locusts. 1. Take 2-5 ml of PBS in a clean
Lipoprotein Isolation - First week
3 Lyophilization. Day 1: Bleeding and centrifugation. Note: You need to wear gloves throughout the procedure. Each groups takes 10-15 locusts. 1. Take 2-5 ml of PBS in a clean 100 ml beaker and add 25 µl of 200 mM PMSF
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