4,4′-(1-苯乙基)双酚,1571-75-1，45045

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

Humes, E. and Yan, H. 2006. Convenient synthesis of 3′,5′‐cyclic diguanylic acid (cdiGMP). Nucleic Acids Symp. Ser. 50:5‐6.    Hyodo, M. and Hayakawa, Y. 2004. An improved method

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

mM triethylammonium acetate (TEAA, pH 7.1); buffer B: 50% acetonitrile in buffer A. Retention times for crude 5′‐TTP and 3′‐TTP after NaCl/ethanol precipitation = 19.4 and 20.7 min, respectively. 3′‐TTP was compared and characterized with a 3′‐TTP

免疫组化的经验总结--操作要点和技巧

′。 3. 脱水， 100 % →95% →85% →75% 酒精，每级均为3 ′。 4. 蒸馏水冲洗， PBS 5 ′� 1 5. 微波炉修复抗原： 0.01M 柠檬酸盐缓冲液， 99 ℃ 肺 20 ′，心肾 12 ′ 6. PBS 3 ′ *3 7. 0.03 % H2 O2 - 甲醇，室温20 ′ 8. PBS 冲洗， PBS 3 ′ *3 ，甩干或纸巾吸去多余液体（勿碰到组织