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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- CAS号:
34513-98-9
- 规格:
250ML

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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Microcentrifuge tubes Micropipettor and tips Rotator Sonicator Spectrometer (Qubit Systems) Test tubes, conical, 50- and 250-mL Thermal cycler Tubes (microcentrifuge) Vortex mixer Water baths or heating
Preparation of BAC DNA with by Alkaline Prep Method
green) or spectromphotometer. Should get 4-7µg per 250ml culture that is 95-98% BAC DNA. 18. Check for E. coli, by loading 2 ml on a 0.8% agarose gel in TAE, run > 63 V for 1 hour. Reagent List 2X YT (Quality Biologicals, 340-023-100) Glycerol stock
green) or spectromphotometer. Should get 4-7µg per 250ml culture that is 95-98% BAC DNA. 17. Check for E. coli, by loading 2 ml on a 0.8% agarose gel in TAE, run > 63 V for 1 hour. Reagent List 10mM EDTA, pH 8.0 10% SDS 5N NaOH ** 7.5M potassium
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