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文献和实验guidelines:Ambion researchers find that siRNAs with 30-50% GC content are more active than those with a higher G/C content.Since a 4-6 nucleotide poly(T) tract acts as a termination signal for RNA pol III, avoid stretches of > 4 T's or A's in the target
Native Chromatin Preparation and Illumina/Solexa Library Construction
. Add 4 µg of RNase A and incubate for 5 min at room temperature. Add 3 µL of 10% SDS and 5 µL of 20 mg/mL proteinase K. Incubate at 65°C for 2 h and purify the DNA by phenol:chloroform extraction and ethanol precipitation. Analyze on a 2% agarose
1 based on the following guidelines: Ambion researchers find that siRNAs with 30-50% GC content are more active than those with a higher G/C content. Since a 4-6 nucleotide poly(T) tract acts as a termination signal for RNA pol III
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