- 询价
- BIO-RAD
- 12004164
- 2025年10月17日
- WB
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
HuCAL Fab monovalent
- 形态：
12004164 A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
- 克隆性：
Monoclonal Antibody
- 标记物：
Rhodamine
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
AbD22606
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
WB
- 抗体名：
Actin antibody | AbD22606
- 规格：
40 µl/200 µl
| 规格： | 40 µl | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 200 µl | 产品价格： | 询价 |
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.
These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.
Features and Benefits
Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.
Features and Benefits
Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
储存信息
1 year at -20°C lyophilized; 6 months at +4°C after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | ✓ | 1/1,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
-
12004164, 12004163
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf
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文献和实验该产品被引用文献
References for Actin antibody
- Seo, T. et al. (2023) MARCH family E3 ubiquitin ligases selectively target and degrade cadherin family proteins.
bioRxiv. Aug 10 [Epub ahead of print]. - Ishiyama, R. et al. (2024) Production of infectious reporter murine * by VP2 trans-complementation.
J Virol. : e0126123. - Garg, S. & , Miousse, I.R. (2024) Rescue of Methionine Dependence by Cobalamin in a Human Colorectal Cancer Cell Line
Nutrients. 16 (7): 997. - Hyder, U. et al. (2024) KAP1 negatively regulates RNA polymerase II elongation kinetics to activate signal-induced transcription
bioRχiv 5 May [Preprint] - Todorova, M.N. et al. (2024) Punica granatum L. leaf extract enhances stress tolerance and promotes healthy longevity through HLH-30/TFEB, DAF16/FOXO, and SKN1/NRF2 crosstalk in Caenorhabditis elegans.
Phytomedicine. 134: 155971. - Smith-Davidson, P. et al. (2024) Prostaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium.
Laryngoscope. Nov 02 [Epub ahead of print]. - Le, K.P.N. et al. (2024) Endogenous ZAP is associated with altered Zika virus infection phenotype
Virology Journal. 21: 285. - Picone, J.A. et al. (2024) The function of the ZFP189 transcription factor in the nucleus accumbens facilitates *-specific transcriptional and behavioral adaptations.
Mol Psychiatry. Nov 25 [Epub ahead of print].
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文献支持
ANTI-ACTIN hFAB™ Rhodamine Antibody，clone AbD22606
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