- 询价
- BIO-RAD
- MCA497PET
- 2025年10月17日
- F, IF
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
- 亚型：
IgG2b
- 形态：
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- 克隆性：
Monoclonal Antibody
- 标记物：
RPE
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
Cl:A3-1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F, IF
- 抗体名：
F4/80 antibody | Cl:A3-1
- 规格：
25 Tests/100 Tests
| 规格： | 25 Tests | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 100 Tests | 产品价格： | 询价 |
Rat anti Mouse F4/80 antibody, clone A3-1 recognizes the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)
Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).
A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)
Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).
A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
储存信息
Pack Size: 25 Tests
This product is shipped at ambient temperature.
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Pack Size: 100 Tests
This product is shipped at ambient temperature.
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | |
| Immunofluorescence | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
Source Reference
- Austyn, J.M. & Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage.
Eur J Immunol. 11 (10): 805-15.
Antibody Characterization Reference
- Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.
Anat Rec. 210 (3): 503-12. - Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
J Exp Med. 161 (3): 475-89.
References for F4/80 antibody
- Warschkau, H. & Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes.
J Immunol. 163 (6): 3409-16. - Moore, K.J. et al. (2000) Divergent response to LPS and bacteria in CD14-deficient murine macrophages.
J Immunol. 165 (8): 4272-80. - Brown, G.D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages.
J Exp Med. 196: 407-12. - Biffi, A. et al. (2004) Correction of metachromatic leukodystrophy in the mouse model by transplantation of genetically modified hematopoietic stem cells.
J Clin Invest. 113: 1118-29. - Dandekar, A.A.et al. (2004) Bystander CD8 T-cell-mediated demyelination is interferon-gamma-dependent in a coronavirus model of multiple sclerosis.
Am J Pathol. 164: 363-9. - Bevaart, L. et al. (2004) CpG oligodeoxynucleotides enhance FcgammaRI-mediated cross presentation by dendritic cells.
Int Immunol. 16: 1091-8. - Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.
J Physiol. 562 (Pt 3): 899-913. - Lin, H.H. et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
J Exp Med. 201 (10): 1615-25.
Further Reading
- Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr Top Microbiol Immunol. 181: 1-37.
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Rat anti Mouse F4/80:RPE，clone Cl:A3-1
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