Mouse anti Rat CD200,clone OX-2
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Mouse anti Rat CD200,clone OX-

2
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  • 询价
  • BIO-RAD已认证
  • MCA44GA
  • 2025年10月17日
  • C, EM, F, IF, IP
  • Rat
  • 详细信息
  • 文献和实验
  • 技术资料
  • 免疫原

    Rat thymocyte membrane glycoproteins.

  • 亚型

    IgG1

  • 形态

    Purified IgG - liquid

  • 克隆性

    Monoclonal Antibody

  • 标记物

    Purified

  • 适应物种

    Rat

  • 保质期

    自发货之日起 12 个月

  • 目录编号

    OX-2

  • 供应商

    伯乐生命医学产品(上海)有限公司

  • 应用范围

    C, EM, F, IF, IP

  • 浓度

    IgG concentration 1.0 mg/ml

  • 抗体名

    CD200 antibody | OX-2

  • 规格

    0.1 mg/0.25 mg

规格:0.1 mg产品价格:询价
规格:0.25 mg产品价格:询价
Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.

The CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation. Mouse anti Rat CD200 antibody, clone OX-2 blocks OX-2 mediated T cell co-stimulation (Ko et al. 2009).

Mouse anti Rat CD200 antibody, clone OX-2 is routinely tested by flow cytometry on rat thymocytes.


储存信息

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.


应用

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.


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图标文献和实验
该产品被引用文献

References for CD200 antibody

  1. Mcmaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
    Eur J Immunol. 9 (6): 426-33.
  2. Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
    Immunology. 52 (4): 631-40.
  3. Barclay, A.N. (1981) Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens.
    Immunology. 44 (4): 727-36.
  4. Webb, M. & Barclay, A.N. (1984) Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
    J Neurochem. 43 (4): 1061-7.
  5. Matsumoto, H. et al. (2007) Expression of CD200 by macrophage-like cells in ischemic core of rat brain after transient middle cerebral artery occlusion.
    Neurosci Lett. 418: 44-8.
  6. Borriello, F. et al. (1997) MRC OX-2 defines a novel T cell costimulatory pathway.
    J Immunol. 158 (10): 4548-54.
  7. Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
    Invest Ophthalmol Vis Sci. 42: 170-6.
  8. Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
    J Anat. 214: 183-95.

Further Reading

  1. Clark, M.J. et al. (1985) MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.
    EMBO J. 4 (1): 113-8.
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Mouse anti Rat CD200,clone OX-2
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