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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Rat thymocyte membrane glycoproteins.
- 亚型:
IgG1
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Rat
- 保质期:
自发货之日起 12 个月
- 目录编号:
OX-2
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, EM, F, IF, IP
- 浓度:
IgG concentration 1.0 mg/ml
- 抗体名:
CD200 antibody | OX-2
- 规格:
0.1 mg/0.25 mg
| 规格: | 0.1 mg | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 0.25 mg | 产品价格: | 询价 |
Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.
The CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation. Mouse anti Rat CD200 antibody, clone OX-2 blocks OX-2 mediated T cell co-stimulation (Ko et al. 2009).
Mouse anti Rat CD200 antibody, clone OX-2 is routinely tested by flow cytometry on rat thymocytes.
The CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation. Mouse anti Rat CD200 antibody, clone OX-2 blocks OX-2 mediated T cell co-stimulation (Ko et al. 2009).
Mouse anti Rat CD200 antibody, clone OX-2 is routinely tested by flow cytometry on rat thymocytes.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/50 | 1/100 |
| Immuno-electron Microscopy | ✓ | ||
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
References for CD200 antibody
- Mcmaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
Eur J Immunol. 9 (6): 426-33. - Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Immunology. 52 (4): 631-40. - Barclay, A.N. (1981) Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens.
Immunology. 44 (4): 727-36. - Webb, M. & Barclay, A.N. (1984) Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
J Neurochem. 43 (4): 1061-7. - Matsumoto, H. et al. (2007) Expression of CD200 by macrophage-like cells in ischemic core of rat brain after transient middle cerebral artery occlusion.
Neurosci Lett. 418: 44-8. - Borriello, F. et al. (1997) MRC OX-2 defines a novel T cell costimulatory pathway.
J Immunol. 158 (10): 4548-54. - Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42: 170-6. - Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
J Anat. 214: 183-95.
Further Reading
- Clark, M.J. et al. (1985) MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.
EMBO J. 4 (1): 113-8.
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Mouse anti Rat CD200,clone OX-2
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