- 询价
- BIO-RAD
- MCA1487
- 2025年10月17日
- E, F *, WB
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant murine TNF-alpha expressed in E. coli.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
MP6-XT3
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
E, F *, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
TNF Alpha antibody | MP6-XT3
- 规格：
0.5 mg
Rat anti Mouse TNF alpha antibody, clone MP6-XT3 recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.
Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Bauman et al. 2003).
Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Bauman et al. 2003).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 5ug/ml | |
| Flow Cytometry 1 | ✓ | ||
| Western Blotting | ✓ |
- 1 Membrane permeabilisation is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
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文献和实验该产品被引用文献
References for TNF Alpha antibody
- Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.Immunol Rev. 127: 5-24.
- Huffnagle, G.B. et al. (1996) Afferent phase production of TNF-alpha is required for the development of protective T cell immunity to Cryptococcus neoformans.
J Immunol. 157: 4529-36. - Bauman, S.K. et al. (2003) Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response.
Infect Immun. 71 (1): 68-74. - Reljic, R. et al. (2004) Mouse IgA inhibits cell growth by stimulating tumor necrosis factor-alpha production and apoptosis of macrophage cell lines.
Int Immunol. 16 (4): 607-14. - Kontogianni, G.I. et al. (2025) A Mechanically Stimulated Co-culture in 3-Dimensional Composite Scaffolds Promotes Osteogenic and Anti-osteoclastogenic Activity and M2 Macrophage Polarization.
Biomater Res. 29: 0135.
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文献支持
Rat anti Mouse TNF Alpha，clone MP6-XT3
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