- 询价
- BIO-RAD
- MCA620R
- 2025年10月17日
- C *, F, IP, WB
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Spleen cell homogenate.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Rat
- 保质期：
自发货之日起 12 个月
- 目录编号：
ED9
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C *, F, IP, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
CD172a antibody | ED9
- 规格：
0.25 mg
Mouse anti Rat CD172a antibody, clone ED9 recognizes rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRPα -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid ~56 kDa single pass type 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons (UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OX-41 (Adams et al. 1998) and has been reported to block the interaction of CD172a - CD47 (de Vries et al. 2002).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/10 | 1/50 |
| Immunohistology - Frozen 1 | ✓ | 1/100 | 1/1000 |
| Immunohistology - Paraffin | × | ||
| Immunohistology - Resin | × | ||
| Immunoprecipitation | ✓ | ||
| Western Blotting | ✓ |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of * fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
References for CD172a antibody
- Damoiseaux, J.G. et al. (1989) Rat bone marrow and monocyte cultures: influence of culture time and lymphokines on the expression of macrophage differentiation antigens.
J Leukoc Biol. 46 (3): 246-53. - Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
J Leukoc Biol. 46 (6): 556-64. - Adams, S. et al. (1998) Signal-regulatory protein is selectively expressed by myeloid and neuronal cells.
J Immunol. 161 (4): 1853-9. - DeVries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
J Immunol. 168 (11): 5832-9. - Blackbeard, J. et al. (2007) Quantification of the rat spinal microglial response to peripheral nerve injury as revealed by immunohistochemical image analysis and flow cytometry.
J Neurosci Methods. 164 (2): 207-17. - Bode, U. et al. (2008) Dendritic cell subsets in lymph nodes are characterized by the specific draining area and influence the phenotype and fate of primed T cells.
Immunology. 123 (4): 480-90. - Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92. - Holler, J. et al. (2008) Neuropeptide Y is expressed by rat mononuclear blood leukocytes and strongly down-regulated during inflammation.
J Immunol. 181 (10): 6906-12.
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Mouse anti Rat CD172a，clone ED9
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