- 询价
- BIO-RAD
- MCA2694T
- 2025年10月17日
- C, F, IF, IP
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Mouse thymus or spleen.
- 亚型：
IgG2a
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
53-6.7
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, F, IF, IP
- 浓度：
IgG concentration 0.5mg/ml
- 抗体名：
CD8 Alpha antibody | 53-6.7
- 规格：
25 µg/0.25 mg
| 规格： | 25 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.25 mg | 产品价格： | 询价 |
Rat anti Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, also known as Lyt-2. CD8α is the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain.
CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules.
Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has also been used for depletion of CD8a+ cells.
CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules.
Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has also been used for depletion of CD8a+ cells.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat Pack Size: 25 µg 1/20 Pack Size: 0.25 mg |
1/20 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
-
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Immunohistology
- MCA2694 has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.
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文献和实验该产品被引用文献
References for CD8 Alpha antibody
- Takahashi, K. et al. (1992) CD4 and CD8 regulate interleukin 2 responses of T cells.
Proc Natl Acad Sci U S A. 89 (12): 5557-61. - Shore, D.A. et al. (2008) The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.
J Mol Biol. 384 (5): 1190-202. - McDole, J.R. et al. (2010) Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells.
Am J Pathol. 177 (4): 1823-33. - Schuster, C. et al. (2013) A missing PD-L1/PD-1 coinhibition regulates diabetes induction by preproinsulin-specific CD8 T-cells in an epitope-specific manner.
PLoS One. 8 (8): e71746. - Johnson, H.L. et al. (2014) Perforin competent CD8 T cells are sufficient to cause immune-mediated blood-brain barrier disruption.
PLoS One. 9 (10): e111401. - Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
J Clin Invest. 125 (11): 4281-94. - Ulaganathan, V.K. & Vasileva, M.H. (2023) A strategy for uncovering germline variants altering anti-tumor CD8 T cell response.
J Genet Genomics. 50 (5): 353-361.
Further Reading
- Jabbari, A. & Harty, J.T. (2006) The generation and modulation of antigen-specific memory CD8 T cell responses.
J Leukoc Biol. 80 (1): 16-23.
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Rat anti Mouse CD8 Alpha，clone 53-6.7
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