- 询价
- BIO-RAD
- 2329-9907
- 2025年10月17日
- C, E, IF, P *
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.
- 亚型：
IgG1
- 形态：
Purified Ig - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
4H9
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, E, IF, P *
- 浓度：
1mg/ml
- 抗体名：
Corticotrophin Releasing Factor antibody | 4H9
- 规格：
0.2 mg
Mouse anti Human Corticotrophin Releasing Factor antibody, clone 4H9 recognizes human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.
The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | ||
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunohistology - Paraffin 1 | ✓ | 1/100 | 1/7000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献和实验该产品被引用文献
References for Corticotrophin Releasing Factor antibody
- Kravchenko, I. V. & Furalev, V. A. (1994) Monoclonal antibodies directed against two different corticotropin-releasing factor determinants.
Hybridoma. 13(1): 59-64. - DeBellis, A. et al. (2012) Involvement of hypothalamus autoimmunity in patients with autoimmune hypopituitarism: role of antibodies to hypothalamic cells.
J Clin Endocrinol Metab. 97 (10): 3684-90.
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文献支持
Mouse anti Human Corticotrophin Releasing Factor，clone 4H9
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