Mouse anti Human CD184，clone 1

Mouse anti Human CD184 clone 12G5, recognizes CD184 also known as C-X-C chemokine receptor 4 (CXCR4) and fusion which is a G-protein-coupled chemokine receptor. It has an extracellular N-terminus, a 7-transmembrane structure with 7 helical regions connected by 6 membrane loops and a cytoplasmic C-terminus. It is expressed in a number of hematopoietic cells including hematopoietic stem cells, T cells, B cells, monocytes, macrophages, neutrophils and eosinophils (Teicher & Fricker 2010).

CD184’s ligand is C-X-C chemokine ligand 12 (CXCL12) which is also known as stromal cell-derived factor 1 (SDF-1). Upon binding of the ligand signaling events are initiated which can result in alterations in gene expression, actin polymerisation, cell skeleton rearrangement and cell migration (Domanska et al. 2013). Via these pathways CD184 plays a role in homing hematopoietic stem and progenitor cells in bone marrow and mobilizes the cells to the periphery during stress or injury (Pawig et al. 2015). As well as homeostatic processes, CD184 has also been linked with various pathologies, including systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis and tumorogenesis. Its expression in CD4+ T cells has also shown that it acts as a natural co-receptor for HIV type I by assisting with triggering membrane fusion of the virus to the target cell (Feng et al. 1996).

Clone 12G5 has been used in flow cytometry experiments to examine CD184 cell surface expression in cells modified to express Δ20 IFNITM2, an IFN-induced transmembrane protein which is known for its ability to inhibit the binding of several human viruses (Wu et al. 2017).