- 询价
- BIO-RAD
- MCA2086
- 2025年10月17日
- F, IP, WB
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peripheral blood mononuclear cells.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
MEM-148
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F, IP, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
CD18 antibody | MEM-148
- 规格：
0.2 mg
Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | ||
| Immunoprecipitation | ✓ | ||
| Western Blotting | ✓ | Non-reducing conditions |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD18 antibody
- Drbal, K. et al. (2001) A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells.
Blood. 98 (5): 1561-6. - Ehirchiou, D. et al. (2005) Dual function for a unique site within the beta2I domain of integrin alphaMbeta2.
J Biol Chem. 280: 8324-31. - Solovjov, D.A. et al. (2005) Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2.
J Biol Chem. 280: 1336-45. - Cairo, C.W. et al. (2006) Cytoskeletal regulation couples LFA-1 conformational changes to receptor lateral mobility and clustering.
Immunity. 25: 297-308. - Shi, M. et al. (2007) A structural hypothesis for the transition between bent and extended conformations of the leukocyte beta2 integrins.
J Biol Chem. 282: 30198-206. - Cheng, M. et al. (2007) Mutation of a conserved asparagine in the I-like domain promotes constitutively active integrins alphaLbeta2 and alphaIIbbeta3.
J Biol Chem. 282: 18225-32. - Kudlová M. et al. (2007) Expression of an activated form of integrin beta2 chain CD18 in cardiac surgical operations.
Acta Medica (Hradec Kralove). 50: 187-93. - Anogianaki, A. et al. (2007) Capsaicin an irritant anti-inflammatory compound.
J Biol Regul Homeost Agents. 21: 1-4.
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文献支持
Mouse anti Human CD18 (Activation Epitope)，clone MEM-148
询价
