- 询价
- BIO-RAD
- MCA155G
- 2025年10月17日
- C, F, IF, P, WB
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
PHA activated rat lymphocytes.
- 亚型：
IgG2a
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Rat
- 保质期：
自发货之日起 12 个月
- 目录编号：
OX-26
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, F, IF, P, WB
- 浓度：
IgG concentration 1 mg/ml
- 抗体名：
CD71 antibody | OX-26
- 规格：
1 mg/0.25 mg
| 规格： | 1 mg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.25 mg | 产品价格： | 询价 |
Mouse anti Rat CD71 antibody, clone OX-26 recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.
The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihton et al. 1992).
An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009).
Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardi et al. 2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure.
The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihton et al. 1992).
An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009).
Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardi et al. 2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/50 Pack Size: 1 mg 1/10 Pack Size: 0.25 mg |
1/200 Pack Size: 1 mg 1/100 Pack Size: 0.25 mg |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunohistology - Paraffin | ✓ | ||
| Western Blotting | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
References for CD71 antibody
- Jefferies, W.A. et al. (1985) Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.
Immunology. 54 (2): 333-41. - Yefimova, M.G. et al. (2002) Impaired retinal iron homeostasis associated with defective phagocytosis in Royal College of Surgeons rats.
Invest Ophthalmol Vis Sci. 43 (2): 537-45. - Jefferies, W.A. et al. (1984) Transferrin receptor on endothelium of brain capillaries.
Nature. 312 (5990): 162-3. - Lipardi, C. et al. (2002) Differential recognition of a tyrosine-dependent signal in the basolateral and endocytic pathways of thyroid epithelial cells.
Endocrinology. 143 (4): 1291-301. - Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
Stem Cells Dev. 18 (10): 1389-98. - Jung, S.H. et al. (2008) Plantaris muscle of aged rats demonstrates iron accumulation and altered expression of iron regulation proteins.
Exp Physiol. 93: 407-14. - Chen, X. et al. (2000) Oxidative damage in an esophageal adenocarcinoma model with rats.
Carcinogenesis. 21: 257-63. - Huang, E. et al. (2009) Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
Acta Histochem Cytochem. 42: 129-36.
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Mouse anti Rat CD71，clone OX-26
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