- 询价
- BIO-RAD
- TZA0104P
- 2025年10月17日
- E
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Daratumumab
- 亚型：
HuCAL Fab monovalent
- 形态：
A bivalent human recombinant Fab (kappa light chain) generated by coupling of the monovalent SpyTagged Fab TZA0104 with a HRP conjugated BiSpyCatcher version 2. The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- 克隆性：
Monoclonal Antibody
- 标记物：
HRP
- 保质期：
自发货之日起 12 个月
- 目录编号：
AbD45015
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
E
- 浓度：
Antibody concentration 0.5 mg/ml
- 抗体名：
Daratumumab antibody | AbD45015
- 规格：
0.1 mg
Human anti daratumumab antibody, clone AbD45015 is a paratope specific, inhibitory anti-idiotypic antibody that recognizes the monoclonal antibody daratumumab. It does not recognize free CD38 nor daratumumab in complex with CD38. The antibody can be used to measure free daratumumab in patient samples.
Human anti daratumumab antibody, clone AbD45015 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA0104. It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 (TZC002P) for use in direct ELISA under product code TZA0104P.
A pair of anti daratumumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, and a bivalent format of the same clone is recommended as the detection antibody. For example, use clone AbD45015ia (HCA364) in full IgG1 format, or convert the monovalent SpyTagged Fab to a bivalent format by coupling to a SpyCatcher product.
Daratumumab (trade name Darzalex) is a human IgG1/kappa antibody generated using transgenic mouse technology. It has been approved for the treatment of patients with multiple myeloma. It has orphan drug designation for multiple myeloma, diffuse large B cell lymphoma, follicular lymphoma, and mantle cell lymphoma. The antibody drug targets CD38, overexpressed by multiple myeloma cells, and indirectly induces apoptosis. In addition, daratumumab modulates CD38 enzymatic activity, inhibiting the cyclase enzyme activity and stimulating the hydrolase activity (Van de Donk et al. 2016).
View a summary of all anti-daratumumab antibodies
Human anti daratumumab antibody, clone AbD45015 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA0104. It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 (TZC002P) for use in direct ELISA under product code TZA0104P.
A pair of anti daratumumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, and a bivalent format of the same clone is recommended as the detection antibody. For example, use clone AbD45015ia (HCA364) in full IgG1 format, or convert the monovalent SpyTagged Fab to a bivalent format by coupling to a SpyCatcher product.
Daratumumab (trade name Darzalex) is a human IgG1/kappa antibody generated using transgenic mouse technology. It has been approved for the treatment of patients with multiple myeloma. It has orphan drug designation for multiple myeloma, diffuse large B cell lymphoma, follicular lymphoma, and mantle cell lymphoma. The antibody drug targets CD38, overexpressed by multiple myeloma cells, and indirectly induces apoptosis. In addition, daratumumab modulates CD38 enzymatic activity, inhibiting the cyclase enzyme activity and stimulating the hydrolase activity (Van de Donk et al. 2016).
View a summary of all anti-daratumumab antibodies
储存信息
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD45015pap can be used as a detection antibody to develop a pharmacokinetic (PK) bridging ELISA for daratumumab, together with TZA0104 or HCA364 as the capture reagent.
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文献和实验该产品被引用文献
Further Reading
- van, N.D.D. et al. (2016) Monoclonal antibodies targeting CD38 in hematological malignancies and beyond.
Immunol Rev. 270 (1): 95-112.
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文献支持
Human anti Daratumumab:HRP，clone AbD45015
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