- 询价
- BIO-RAD
- MCA2385F
- 2025年10月17日
- F
- Horse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Equine PBMCs.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Horse
- 保质期：
自发货之日起 12 个月
- 目录编号：
CVS8
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD8 antibody | CVS8
- 规格：
0.1 mg
Mouse anti Horse CD8, clone CVS8 is a monoclonal antibody recognising the equine homologue of the human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.
A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007).
A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD8 antibody
- Lunn, D.P et al (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets
Immunology 74: 251-257. - Lunn, D.P. et al. (1998) Report of the second equine leucocyte antigen workshop, Squaw Valley, California July 1995.
Vet Immunol Immunopathol. 62: 101-143. - Merant, C. et al. (2003) Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry.
Vet Res. 34: 791-801. - Ibrahim, S (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28
Chapter 5 in PhD Thesis Freie Universität Berlin - Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
Can J Vet Res. 71: 213-7. - Jacks, S.
(2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
Clin Vaccine Immunol. 14: 669-77. - Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
Vet Immunol Immunopathol. 199: 22-31. - Carossino, M. et al. (2019) Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis.
PLoS Pathog. 15 (7): e1007950.
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文献支持
Mouse anti Horse CD8:FITC，clone CVS8
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