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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human T lymphocytes
- 亚型:
IgG1
- 形态:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Human
- 保质期:
自发货之日起 12 个月
- 目录编号:
F8-11-13
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
IgG concentration 0.1 mg/ml
- 抗体名:
CD45RA antibody | F8-11-13
- 规格:
0.1 mg
Mouse anti Human CD45RA antibody, clone F8-11-13 recognizes the human CD45RA cell surface antigen.
CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.
Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.
Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul lysed whole blood
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文献和实验该产品被引用文献
References for CD45RA antibody
- Dalchau, R. & Fabre, J.W. (1981) Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule.
J Exp Med. 153 (4): 753-65. - O'Grady, J.T. et al. (1994) CD40 expression in Hodgkin's disease.
Am J Pathol. 144 (1): 21-6. - Franitza, S. et al. (2002) TGF-beta1 enhances SDF-1alpha-induced chemotaxis and homing of naive T cells by up-regulating CXCR4 expression and downstream cytoskeletal effector molecules.
Eur J Immunol. 32 (1): 193-202. - Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of *-infected persons with oropharyngeal Candidiasis.
Clin Vaccine Immunol. 13 (6): 678-83. - Hunt, L. et al. (2016) T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals.
Ann Rheum Dis. 75 (10): 1884-9. - Ibana, J.A. et al. (2012) The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.
BMC Immunol. 13: 66. - Ponchel, F. et al. (2014) An immunological biomarker to predict MTX response in early RA.
Ann Rheum Dis. 73 (11): 2047-53.
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Mouse anti Human CD45RA:FITC,clone F8-11-13
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