- 询价
- BIO-RAD
- MCA116F
- 2025年10月17日
- F
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Immune complex precipitated from an HLA-B27 positive cell line by anti HLA antibody and staphylococcal protein A.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
HLA-ABC-m3
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
50 ug/ml
- 抗体名：
HLA B27 antibody | HLA-ABC-m3
- 规格：
100 Tests/1ml
Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 recognizes the HLA-B27 alloantigen and reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kDa, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
Scatchard affinity analysis shows that Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).
Flow cytometry - subjects expected fluorescence intensity:
Heterozygous HLA-B27+ = Strong.
Homozygous HLA-B27+ = Strong.
Heterozygous HLA-B7+ = Faint.
Non B27, Non B7 = Negative.
Scatchard affinity analysis shows that Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).
Flow cytometry - subjects expected fluorescence intensity:
Heterozygous HLA-B27+ = Strong.
Homozygous HLA-B27+ = Strong.
Heterozygous HLA-B7+ = Faint.
Non B27, Non B7 = Negative.
储存信息
This product is shipped at ambient temperature.
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood.
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文献和实验该产品被引用文献
Source Reference
- Trapani, J.A. et al. (1983) Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
Hum Immunol. 7 (4): 205-16.
References for HLA B27 antibody
- Orr, K. et al. (1994) Utilization of commercial antisera and flow cytometry in HLA-B27 typing.
Cytometry (Comm. Clin. Cytometry) 18: 17-20. - Levering, W.H. et al. (2003) Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens.
Cytometry B Clin Cytom. 54: 28-38. - Mandic, R. et al. (2004) Comparison of surface HLA class I levels in squamous cell carcinoma cell lines of the head and neck.
Anticancer Res. 24 (2B): 973-9. - Meyer, O. et al. (2006) A simple and practical agglutination assay for human leucocyte antigen-B27 typing.
Vox Sang. 91 (1): 77-80. - Goodall, J.C. et al. (2007) Does HLA-B27 influence the monocyte inflammatory response to lipopolysaccharide?
Rheumatology (Oxford). 46: 232-7. - Meyer, O. et al. (2008) Application of the particle gel agglutination assay in the typing of single human leucocyte antigens.
Tissue Antigens. 71 (2): 157-9. - Marroquin, B. O et al. (2015) HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.
PLoS One. 10 (6): e0130811.
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文献支持
Mouse anti Human HLA B27:FITC，clone HLA-ABC-m3
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