- 询价
- BIO-RAD
- MCA5935F
- 2025年10月17日
- F, IF
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant soluble JAM-C.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
CRAM-18 F26
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F, IF
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
JAM-C antibody | CRAM-18 F26
- 规格：
0.1 mg
Rat anti Mouse JAM-c antibody, clone CRAM-18 F26 recognizes mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.
JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.
Rat anti Mouse JAM-c antibody, clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Léger et al. 2002).
JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.
Rat anti Mouse JAM-c antibody, clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Léger et al. 2002).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/5 |
| Immunofluorescence | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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文献和实验该产品被引用文献
References for JAM-C antibody
- Aurrand-lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
J Biol Chem. 276 (4): 2733-41. - Aurrand-lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.
Blood. 98 (13): 3699-707. - Johnson-léger, C.A. et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
Blood. 100 (7): 2479-86. - Forsberg, E.C. et al. (2005) Differential expression of novel potential regulators in hematopoietic stem cells.
PLoS Genet. 1(3):e28. - Radulovic, V. et al. (2019) Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis.
Cell Rep. 27 (10): 2826-2836.e5. - Miranda, J. et al. (2019) Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.
Cells. 8 (10)Sep 29 [Epub ahead of print].
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文献支持
Rat anti Mouse JAM-C:FITC，clone CRAM-18 F26
询价
