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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Ovine leucocytes.
- 亚型:
IgG2a
- 形态:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Sheep
- 保质期:
自发货之日起 12 个月
- 目录编号:
CO.3E1D4
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
IgG concentration 0.1 mg/ml
- 抗体名:
CD31 antibody | CO.3E1D4
- 规格:
0.1 mg
Mouse anti Sheep CD31 antibody, clone CO.3E1D4 recognizes ovine CD31, also known as PECAM-1.
Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.
Mouse anti Sheep CD31 antibody, clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies (Pintado et al. 1995).
Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.
Mouse anti Sheep CD31 antibody, clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies (Pintado et al. 1995).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
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文献和实验该产品被引用文献
Source Reference
- Pintado. C. O. et al. (1995) A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti CD43 monoclonal antibodies of ruminant leukocytes.
Immunol. Lett. 45: 81 - 85.
References for CD31 antibody
- Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species:
Vet. Immunol. Immunopathol. 64: 1-13. - Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
Immunology. 113: 310-7. - Summers, C. et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 106 (3-4): 285-94. - Zannettino, A.C. et al. (2010) Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells.
Tissue Eng Part A. 16 (12): 3579-87. - De Visscher, G. et al. (2010) Selection of an immunohistochemical panel for cardiovascular research in sheep.
Appl Immunohistochem Mol Morphol. 18: 382-91. - Filby, C.E. et al. (2010) Partial pulmonary embolization disrupts alveolarization in fetal sheep.
Respir Res. 11: 42. - Boos, A.M. et al. (2011) Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model.
J Cell Mol Med. 15 (6): 1364-78. - Berardinelli, P. et al. (2013) Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation.
Clin Oral Investig. 17 (7): 1661-75.
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Mouse anti Sheep CD31:FITC,clone CO.3E1D4
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