- 询价
- BIO-RAD
- MCA2483T
- 2025年10月17日
- C, F *, IC *, IF, P
- Chemical
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Bromodeoxyuridine conjugated to BSA
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Chemical
- 保质期：
自发货之日起 12 个月
- 目录编号：
Bu20a
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, F *, IC *, IF, P
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
BrdU antibody | Bu20a
- 规格：
20 µg/0.1 mg/0.2 mg
| 规格： | 20 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.1 mg | 产品价格： | 询价 |
| 规格： | 0.2 mg | 产品价格： | 询价 |
Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989).
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | ✓ | 1/25 | 1/100 |
| Immunocytochemistry 2 | ✓ | 1/25 | 1/100 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunohistology - Paraffin | ✓ |
- 1 Flow Cytometry protocols can be found at:
www.bio-rad-antibodies.com/brdu-clone-bu20a-flow-cytometry-protocol
www.bio-rad-antibodies.com/brdu-staining-cell-cycle-protocol - 2 BrdU labeling and immunostaining protocol can be found at:
www.bio-rad-antibodies.com/brdu-labeling-and-immunostaining-protocol
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
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文献和实验该产品被引用文献
Source Reference
- Magaud, J.P. et al. (1989) Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
J Histochem Cytochem. 37 (10): 1517-27.
References for BrdU antibody
- Xie, L.L. et al. (2009) Aquaporin 4 knockout resists negative regulation of neural cell proliferation by * in mouse hippocampus.
Int J Neuropsychopharmacol. 12 (6): 843-50. - Wohl, S.G. et al. (2009) Optic nerve lesion increases cell proliferation and nestin expression in the adult mouse eye in vivo.
Exp Neurol. 219 (1): 175-86. - Innis, S.M. et al. (2010) Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.
Am J Physiol Gastrointest Liver Physiol. 299 (6): G1376-85. - Caronia, G. et al. (2010) Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior.
J Neurosci. 30: 6291-301. - Miller, C. et al. (2011) The interplay between SUCLA2, SUCLG2, and mitochondrial DNA depletion.
Biochim Biophys Acta. 1812 (5): 625-9. - Sato, Y. et al. (2013) Grafting of neural stem and progenitor cells to the hippocampus of young, irradiated mice causes gliosis and disrupts the granule cell layer.
Cell Death Dis. 4: e591. - Pappalardo, L.W. et al. (2014) Voltage-gated sodium channel Nav 1.5 contributes to astrogliosis in an in vitro model of glial injury via reverse Na+ /Ca2+ exchange.
Glia. 62 (7): 1162-75. - Kent BA et al. (2015) The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 51: 431-9.
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Mouse anti BrdU，clone Bu20a
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