- 询价
- BIO-RAD
- MCA771FT
- 2025年10月17日
- F
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Cultured bone marrow cells
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
7/4
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
MCA771FT, MCA771FA, MCA771F: IgG concentration 0.1 mg/ml
- 抗体名：
Ly-6B.2 Alloantigen antibody | 7/4
- 规格：
25 µg/0.5 mg/50 µg/0.1 mg
| 规格： | 25 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.5 mg | 产品价格： | 询价 |
| 规格： | 50 µg | 产品价格： | 询价 |
| 规格： | 0.1 mg | 产品价格： | 询价 |
Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosas et al.2010).
In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).
In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).
储存信息
Pack Size: 25 µg, 50 µg, 0.1 mg
This product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.5 mg
This product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat Pack Size: 25 µg, 0.1 mg 1/5 Pack Size: 0.5 mg |
1/10 Pack Size: 25 µg, 0.1 mg 1/50 Pack Size: 0.5 mg |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
Source Reference
- Hirsch, S. & Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4.
Immunogenetics. 18 (3): 229-39.
References for Ly-6B.2 Alloantigen antibody
- Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr Top Microbiol Immunol. 181: 1-37. - Horiba, M. et al. (2000) Neointima formation in a restenosis model is suppressed in midkine-deficient mice.
J Clin Invest. 105: 489-95. - Pyo, R. et al. (2000) Targeted gene disruption of matrix metalloproteinase-9 (gelatinase B) suppresses development of experimental abdominal aortic aneurysms.
J Clin Invest. 105: 1641-9. - Liao, C. et al. (2001) Altered myelopoiesis and the development of acute myeloid leukemia in transgenic mice overexpressing cyclin A1.
Proc Natl Acad Sci U S A. 98: 6853-8. - Song, Y. et al. (2001) A low level of TNF-alpha mediates hemorrhage-induced acute lung injury via p55 TNF receptor.
Am J Physiol Lung Cell Mol Physiol. 281: L677-84. - Singbartl, K. et al. (2001) Platelet, but not endothelial, P-selectin is critical for neutrophil-mediated acute postischemic renal failure.
FASEB J. 15: 2337-44. - Endlich, B. et al. (2002) Distinct temporal patterns of macrophage-inflammatory protein-2 and KC chemokine gene expression in surgical injury.
J Immunol. 168: 3586-94. - Lacroix-Lamandé, S. et al. (2002) Role of gamma interferon in chemokine expression in the ileum of mice and in a murine intestinal epithelial cell line after Cryptosporidium parvum infection.
Infect Immun. 70: 2090-9.
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Rat anti Mouse Ly-6B.2 Alloantigen:FITC，clone 7/4
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