Mouse anti Pig Granulocytes:FITC,clone 6D10
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Mouse anti Pig Granulocytes:FI

TC,clone 6D10
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  • 询价
  • BIO-RAD已认证
  • MCA2599F
  • 2025年10月17日
  • F
  • Pig
  • 详细信息
  • 文献和实验
  • 技术资料
  • 免疫原

    Porcine bone marrow haematopoietic cells (BMHC).

  • 亚型

    IgG2a

  • 形态

    Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

  • 克隆性

    Monoclonal Antibody

  • 标记物

    FITC

  • 适应物种

    Pig

  • 保质期

    自发货之日起 12 个月

  • 目录编号

    6D10

  • 供应商

    伯乐生命医学产品(上海)有限公司

  • 应用范围

    F

  • 浓度

    IgG concentration 0.1mg/ml

  • 抗体名

    Granulocytes antibody | 6D10

  • 规格

    0.1 mg

Mouse anti Pig Granulocytes antibody, clone 6D10 recognizes a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.

Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils.

Mouse anti Pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins (Pérez et al. 2007).


储存信息

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.


应用

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.


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图标文献和实验
该产品被引用文献

References for Granulocytes antibody

  1. Pérez, C. et al. (2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.
    Dev Comp Immunol. 31 (3): 296-306.
  2. Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
    Dev Comp Immunol. 33 (3): 284-98.
  3. Stone, J.P. et al. (2016) Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes Using Ex Vivo Lung Perfusion.
    Am J Transplant. 16 (1): 33-43.
  4. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  5. Nguyen, D.N. et al. (2016) Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
    Sci Rep. 6: 36816.
  6. Andersen, A.D. et al. (2019) Synbiotics Combined with Glutamine Stimulate Brain Development and the Immune System in Preterm Pigs.
    J Nutr. 149 (1): 36-45.
  7. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
  8. dos Santos, M.C. et al. (2023) Effect of yeast extracted β-glucans on the immune response and reproductive performance of gilts in the adaptation, gestation, and lactation periods
    Livestock Sci. 275: 105289.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
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Mouse anti Pig Granulocytes:FITC,clone 6D10
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