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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide containing 20 amino acid residues from mouse CD79b conjugated to Keyhole Limpet Hemocyanin (KLH).
- 亚型:
IgG1
- 形态:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
AT107-2
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F *
- 浓度:
IgG concentration 0.1 mg/ml
- 抗体名:
CD79b antibody | AT107-2
- 规格:
0.1 mg
Rat anti Mouse CD79b antibody, clone AT107-2 recognizes a cytoplasmic region of mouse B-cell antigen receptor complex-associated protein beta chain, also known as B-cell-specific glycoprotein B29, Ig-beta, or Immunoglobulin-associated B29 protein. CD79b is a 228 amino acid, including a 26 signal peptide ~40 kDa type I single pass transmembrane glycoprotein. CD79b is expressed by B lymphocytes and associates with CD79a to form a heterodimer, non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. Rat anti Mouse CD79b antibody, clone AT107-2 also recognizes a homologous region of human CD79b.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | ✓ | 1/50 | 1/100 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
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文献和实验该产品被引用文献
References for CD79b antibody
- Vendel,A.C. et al (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.
J Immunol. 182: 1509-17 - Kreiss, A. et al. (2009) A histological and immunohistochemical analysis of lymphoid tissues of the Tasmanian devil.
Anat Rec (Hoboken). 292: 611-20. - Dornan, D. et al. (2009) Therapeutic potential of an anti-CD79b antibody-drug conjugate, anti-CD79b-vc-MMAE, for the treatment of non-Hodgkin lymphoma.
Blood. 114: 2721-9. - Lau, Q. et al. (2012) Expression and in vitro upregulation of MHCII in koala lymphocytes.
Vet Immunol Immunopathol. 147: 35-43. - Thalheim, L. et al. (2013) Lymphoma immunophenotype of dogs determined by immunohistochemistry, flow cytometry, and polymerase chain reaction for antigen receptor rearrangements.
J Vet Intern Med. 27 (6): 1509-16. - McCurdy, P. et al. (2014) Acute lymphoblastic leukemia in a pygmy hippopotamus (Hexaprotodon liberiensis).
J Zoo Wildl Med. 45 (4): 906-10. - Kandasamy S et al. (2014) Prenatal vitamin A deficiency impairs adaptive immune responses to pentavalent rotavirus vaccine (RotaTeq®) in a neonatal gnotobiotic pig model.
Vaccine. 32 (7): 816-24. - Ouyang, K. et al. (2015) Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under field conditions.
Vet Res. 46: 140.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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Rat anti Mouse CD79b:FITC,clone AT107-2
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