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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human thymic clone B12.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
BB27
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD101 antibody | BB27
- 规格：
0.1 mg
Mouse anti Human CD101 antibody, clone BB27 recognizes human CD101, also known as Immunoglobulin superfamily member 2 (IgSF2), . Cell surface glycoprotein V7, Glu-Trp-Ile EWI motif-containing protein 101 or EWI-101. CD101 is a 1021 amino acid, including a 20 amino acid signal peptide, ~140 kDa single pass type I homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes.
CD101 plays a major role in the activation of T cells by skin dendritic cells. Mouse anti Human CD101 antibody, clone BB27 has been reported to inhibit allogeneic T-cell responses (Bagot et al. 1997).
CD101 plays a major role in the activation of T cells by skin dendritic cells. Mouse anti Human CD101 antibody, clone BB27 has been reported to inhibit allogeneic T-cell responses (Bagot et al. 1997).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/5 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cell in 100μl.
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文献和实验该产品被引用文献
Source Reference
- Gouttefangeas, C. et al. (1994) Differential proliferative responses in subsets of human CD28+ cells delineated by BB27 mAb.
Int Immunol. 6 (3): 423-30.
References for CD101 antibody
- Bagot, M. et al. (1997) CD101 is expressed by skin dendritic cells. Role in T-lymphocyte activation.
Tissue Antigens. 50 (5): 439-48. - Grassi, F. et al. (1998) Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules.
J Leukoc Biol. 64 (4): 484-93. - Jovanovic, D.V. et al. (2011) CD101 expression and function in normal and rheumatoid arthritis-affected human T cells and monocytes/macrophages.
J Rheumatol. 38: 419-28. - Ohradanova-Repic, A. et al. (2016) Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel.
Clin Transl Immunology. 5 (1): e55. - Okuno, M. et al. (2017) Nucleotide substitutions in CD101, the human homolog of a diabetes susceptibility gene in non-obese diabetic mouse, in patients with type 1 diabetes.
J Diabetes Investig. 8 (3): 286-94.
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文献支持
Mouse anti Human CD101:FITC，clone BB27
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