- 询价
- BIO-RAD
- MCA2213F
- 2025年10月17日
- F
- Sheep
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Fetal thymocytes.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Sheep
- 保质期：
自发货之日起 12 个月
- 目录编号：
44.38
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD4 antibody | 44.38
- 规格：
0.1 mg
Mouse anti Sheep CD4 antibody, clone 44.38 recognizes the ovine CD4 cell surface glycoprotein, expressed by a subset of mature T lymphocytes. Mouse anti Sheep CD4 antibody, clone 44.38 immunoprecipitates a protein of ~56 kDa under reducing conditions.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD4 antibody
- Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur J Immunol. 16 (1): 19-25. - Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J Immunol. 136 (5): 1592-9. - Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell Immunol. 110 (1): 46-55. - Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
Nat Immunol. 6: 889-94. - Foulon, E. et al. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339: 1-10. - Umeshappa, C.S. et al. (2010) Cell-mediated immune response and cross-protective efficacy of binary ethylenimine-inactivated bluetongue virus serotype-1 vaccine in sheep.
Vaccine. 28: 2522-31. - Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
Clin Vaccine Immunol. 17: 108-17. - Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22.
Further Reading
- Lybeck, K. R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin. Vaccine. Immunol. 16: 1003-11.
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文献支持
Mouse anti Sheep CD4:FITC，clone 44.38
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