- 询价
- BIO-RAD
- MCA6150F
- 2025年10月17日
- F
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Pig alveolar macrophages
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
3A8
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD11c antibody | 3A8
- 规格：
0.1 mg
Mouse anti Pig CD11c, clone 3A8 recognizes CD11c also known as integrin alpha-X.
Porcine CD11c is mainly expressed by blood and skin polymorphonuclear and mononuclear phagocytes as well as monocytes, granulocytes and alveolar and inflammatory macrophages. Porcine CD11c is not expressed by plasmacytoid dendritic cells (pDC).
Mouse anti Pig CD11c, clone 3A8 antibody can be used to distinguish resident from migrating dendritic cells (DC) in lymph nodes;migrated DC express lower levels of surface CD11c and have higher MHC class II expression than resident DC. Both porcine cDC1 and cDC2 subsets express CD11c, with cDC1 appearing to have slightly lower levels than cDC2. Clone 3A8 did not show species cross-reactivity with human CD11c-CD18 transfectants or sheep myeloid cells (Deloizy et al. 2016).
Porcine CD11c is mainly expressed by blood and skin polymorphonuclear and mononuclear phagocytes as well as monocytes, granulocytes and alveolar and inflammatory macrophages. Porcine CD11c is not expressed by plasmacytoid dendritic cells (pDC).
Mouse anti Pig CD11c, clone 3A8 antibody can be used to distinguish resident from migrating dendritic cells (DC) in lymph nodes;migrated DC express lower levels of surface CD11c and have higher MHC class II expression than resident DC. Both porcine cDC1 and cDC2 subsets express CD11c, with cDC1 appearing to have slightly lower levels than cDC2. Clone 3A8 did not show species cross-reactivity with human CD11c-CD18 transfectants or sheep myeloid cells (Deloizy et al. 2016).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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文献和实验该产品被引用文献
Antibody Characterization Reference
- Deloizy, C. et al. (2016) Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
Dev Comp Immunol. 65: 31-40.
References for CD11c antibody
- Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16) :2103.
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文献支持
Mouse anti Pig CD11c:FITC，clone 3A8
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