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- 2025年10月17日
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human thymic epithelial cells.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
3A6
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1mg/ml
- 抗体名：
CD166 antibody | 3A6
- 规格：
25 µg/0.1 mg
| 规格： | 25 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.1 mg | 产品价格： | 询价 |
Mouse anti Human CD166 antibody, clone 3A6 recognizes the 100 kDa adhesion molecule CD166, also known as ALCAM. CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for ALCAM (Skonier et al. 1996).
Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction with CD44 which is expressed by this cell lineage and the hematopoietic cell marker CD45 which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguez et al. 2017).
Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction with CD44 which is expressed by this cell lineage and the hematopoietic cell marker CD45 which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguez et al. 2017).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD166 antibody
- Patel, D. D. et al. (1997) CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6.
In Leukocyte Typing IV. Kishimoto, T. et al. eds Garland publishing Inc. New York p. 461-4. - Wang, D. et al. (2004) Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor beta1 stimulation.
J Biol Chem. 279 (42): 43725-34. - Yeh, S.P. et al. (2005) Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolonged ex vivo culture.
Leukemia. 19: 1505-7. - Tondreau, T. et al. (2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.
BMC Genomics. 9:166. - Srouji, S. et al. (2009) The Schneiderian membrane contains osteoprogenitor cells: in vivo and in vitro study.
Calcif Tissue Int. 84 (2): 138-45. - Agha-Hosseini, F. et al. (2010) In vitro isolation of stem cells derived from human dental pulp.
Clin Transplant. 24: E23-8. - Bhattacharya, S. et al. (2010) Toponome imaging system: in situ protein network mapping in normal and cancerous colon from the same patient reveals more than five-thousand cancer specific protein clusters and their subcellular annotation by using a three symbol code.
J Proteome Res. 9: 6112-25. - Katsube, Y. et al. (2010) Restoration of cellular function of mesenchymal stem cells from a hypophosphatasia patient.
Gene Ther. 17 (4): 494-502.
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Mouse anti Human CD166:FITC，clone 3A6
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