- 询价
- BIO-RAD
- MCA1521F
- 2025年10月17日
- F
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
L cells transfected with CD40L.
- 亚型：
IgG
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
39H5
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD154 antibody | 39H5
- 规格：
0.1 mg
Hamster anti Mouse CD154 antibody, clone 39H5 recognizes the murine CD40 ligand, also known as CD40L or CD154. CD154 is a ~39 kDa cell surface glycoprotein . CD154 is expressed by activated CD4+ve T lymphocytes.
Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.
Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
-
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
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文献和实验该产品被引用文献
References for CD154 antibody
- Roy, A. et al. (2001) Increased efficiency of gamma-irradiated versus mitomycin C-treated feeder cells for the expansion of normal human cells in long-term cultures
J Hematother Stem Cell Res. 2001 Dec;10(6): 873-80. - Dong, L. et al. (2003) An immunostimulatory oligodeoxynucleotide containing a cytidine-guanosine motif protects senescence-accelerated mice from lethal influenza virus by augmenting the T helper type 1 response
J Gen Virol. 84: 1623-8. - Hogg, K.G. et al. (2003) IL-10 regulates early IL-12-mediated immune responses induced by the radiation-attenuated schistosome vaccine.
Int Immunol. 15 (12): 1451-9. - McGregor, C.M. et al. (2004) CD154 is a negative regulator of autoaggressive CD8+ T cells in type 1 diabetes.
Proc Natl Acad Sci U S A. 101 (25): 9345-50. - Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
Kidney Int. 67 (4): 1340-9. - Neron. S, et al. (2005) Differential responses of human B-lymphocyte subpopulations to graded levels of CD40-CD154 interaction.
Immunology. 2005 Dec;116(4):454-63. - Hacker, U.T. et al. (2006) Gene transfer preferentially selects MHC class I positive tumour cells and enhances tumour immunogenicity.
Cancer Immunol Immunother. 55 (5): 547-57. - Serba, S. et al. (2008) Transfection with CD40L induces tumour suppression by dendritic cell activation in an orthotopic mouse model of pancreatic adenocarcinoma.
Gut. 57 (3): 344-51.
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文献支持
Hamster anti Mouse CD154:FITC，clone 39H5
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